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Normalizes ChIP-seq data using exogenous spike-in (ChIP-Rx with Drosophila chromatin per Orlando 2014 / Egan 2016; E. coli carryover for CUT&RUN/CUT&Tag). Distinguishes RRPM from Rx-Input scaling, integrates with DiffBind / DESeq2 / edgeR / csaw via sizeFactors and DiffBind library-size vectors, and applies the Patel et al 2024 *Nat Biotechnol* review's failure-mode framework to validate that norm

Use when receiving code review feedback, before implementing suggestions, especially if feedback seems unclear or technically questionable - requires technical rigor and verification, not performative agreement or blind implementation

Build Retrieval-Augmented Generation (RAG) systems for LLM applications with vector databases and semantic search. Use when implementing knowledge-grounded AI, building document Q&A systems, or integrating LLMs with external knowledge bases.

AI-powered design of armored CAR-T cells with cytokine/chemokine expression for enhanced solid tumor efficacy, including IL-12, IL-15, IL-18, and IL-7 armoring strategies.

Downloads videos from YouTube and other platforms for offline viewing, editing, or archival. Handles various formats and quality options.

Run remote BLAST searches against NCBI databases using Biopython Bio.Blast. Use when identifying unknown sequences, finding homologs, or searching for sequence similarity against NCBI's nr/nt databases.

Security architecture and implementation patterns. Use when designing

Analise e gerencie permissões de skill, suportando autorização única e em lote.

Evaluate systems through an entropy/negentropy lens — is this system decaying or growing? Use when making architecture decisions, evaluating system health, or when the user mentions "entropy", "negentropy", "technical debt", "system decay", or "tacit knowledge".

Firebase gives you a complete backend in minutes - auth, database, storage, functions, hosting. But the ease of setup hides real complexity. Security rules are your last line of defense, and they're often wrong. Firestore queries are limited, and you learn this after you've designed your data model. This skill covers Firebase Authentication, Firestore, Realtime Database, Cloud Functions, Cloud St

Master REST and GraphQL API design principles to build intuitive, scalable, and maintainable APIs that delight developers. Use when designing new APIs, reviewing API specifications, or establishing API design standards.

Initializes a new Convex project from scratch or adds Convex to an existing app. Use this skill when starting a new project with Convex, scaffolding with npm create convex@latest, adding Convex to an existing React, Next.js, Vue, Svelte, or other frontend, wiring up ConvexProvider, configuring environment variables for the deployment URL, or running npx convex dev for the first time, even if the u