Biochemistry fundamentals for computational biologists, covering Beer-Lambert law, spectrophotometric assays, Michaelis-Menten kinetics, linearization methods, and inhibition models.
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A = ε · l · c
| Symbol | Meaning | Typical value |
|---|---|---|
| A | Absorbance (dimensionless) | 0.1–1.5 (linear range) |
| ε | Molar absorption coefficient (M⁻¹ cm⁻¹) | NADH at 340 nm: 6,220 |
| l | Path length (cm) | 1 cm (standard cuvette) |
| c | Concentration (M) | — |
# Beer-Lambert: absorbance -> concentration
epsilon_NADH = 6220 # M^-1 cm^-1 at 340 nm
path_length = 1 # cm
def a
[Description truncada. Veja o README completo no GitHub.]