Genetic Engineering In Silico
Restriction Enzyme Reference
| Overhang type | Example | Note |
|---|---|---|
| 5' sticky | EcoRI (GAATTC, cut 1/5) | Most common for cloning |
| 3' sticky | KpnI (GGTACC, cut 5/1) | Less common |
| Blunt | SmaI (CCCGGG, cut 3/3) | Ligation-inefficient |
Compatible end rule: same overhang sequence = ligatable (e.g. BamHI + BglII both give 5'-GATC).
Core Functions
import re
RESTRICTION_ENZYMES = {
# name: (recognition_
[Description truncada. Veja o README completo no GitHub.]