scRNA-seq: Trajectory Analysis and RNA Velocity
Method Overview
| Method | Tool | Input | Output |
|---|---|---|---|
| Diffusion pseudotime (DPT) | scanpy sc.tl.dpt | k-NN graph + root cell | Pseudotime per cell |
| PAGA | scanpy sc.tl.paga | Cluster labels + k-NN graph | Cluster connectivity graph |
| RNA velocity (steady-state) | scVelo mode='stochastic' | Spliced + unspliced counts | Velocity arrows |
| RNA velocity (dynamical) | scVelo mode='dynamical' | Splic |
[Description truncada. Veja o README completo no GitHub.]