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bio-single-cell-lineage-tracing
Reconstruct cell lineage trees from CRISPR barcode tracing or mitochondrial mutations. Use when studying clonal dynamics, cell fate decisions, or developmental trajectories.
bio-single-cell-markers-annotation
Find marker genes and annotate cell types in single-cell RNA-seq using Seurat (R) and Scanpy (Python). Use for differential expression between clusters, identifying cluster-specific markers, scoring gene sets, and assigning cell type labels. Use when finding marker genes and annotating clusters.
bio-small-rna-seq-differential-mirna
Perform differential expression analysis of miRNAs between conditions using DESeq2 or edgeR with small RNA-specific considerations. Use when identifying miRNAs that change between treatment groups, disease states, or developmental stages.
bio-sra-data
Download raw sequencing reads from NCBI SRA using sra-tools (prefetch, fasterq-dump, vdb-validate) or the ENA mirror. Use when pulling FASTQ for SRR/ERR/DRR accessions, deciding between SRA-direct, ENA mirror, or AWS/GCP cloud mirror (STRIDES), handling --include-technical for 10x and other single-cell records, validating with MD5/vdb-validate, navigating SRR/SRX/SRS/SRP/PRJNA hierarchy, or findin
use-cases-page-generator
When the user wants to create, optimize, or audit use case pages. Also use when the user mentions "use cases," "use case page," "for [role]," "by persona," "by scenario," "by business goal," "ICP pages," or "audience-specific pages." For sitewide page planning, use website-structure.
commit-author
Generate concise Conventional Commits messages from a staged diff. Enforces full type set, imperative subject lines, no AI co-author attribution. Use when user wants to commit, asks for a commit message, mentions "commit", or invokes /commit.
alchemy-infra
Sets up Alchemy (Infrastructure-as-TypeScript) in any codebase, either for new projects or existing applications. It configures Cloudflare/AWS providers, state backend, secrets, and binding types end-to-end with strict secret hygiene.
midnight-ops-doctor
This diagnostic playbook addresses Midnight Network backend operational issues, including wallet sync stalls, deployment hangs, three-address confusion, DUST zero-balance, cloud-IP RPC 403 errors, and AWS WAF 8KB submitTx problems.
imap-client
Read, search, and download emails over IMAP from the command line using the `myl` CLI client. This skill allows users to interact with their mailbox from a terminal, including checking the inbox, listing/searching messages, reading emails, opening HTML/raw source, or saving attachments.
mmmmealplan
Generates a personalized weekly meal plan and Migros shopping list based on user preferences, utilizing the Migros MCP for promotions and nutrition verification. It can be triggered by commands such as 'meal plan' or 'grocery list for the week'.
cloudwatch
Debug production issues and monitor AWS infrastructure using CloudWatch. This skill is used when users report errors, investigate production behavior, check logs, debug OAuth, API errors, ECS tasks, database issues, WAF blocks, or any production incident, including phrases like "check logs", "what's failing", or "system status".
ai-ml
· Build/review AI apps: LLMs, RAG, embeddings, agents, evals, local inference. Triggers: 'llm', 'rag', 'embedding', 'openai sdk', 'agent loop', 'fine-tune', 'ollama', 'vllm'. Not for MCP (use mcp).