Microbial Diversity Analysis
16S rRNA Primer Targets
| Region | Primers | Length | Notes | |--------|---------|--------|-------| | V4 | 515F/806R | ~250 bp | Earth Microbiome Project standard | | V3-V4 | 341F/785R | ~460 bp | Good taxonomic resolution | | V1-V3 | 27F/534R | ~500 bp | Alternative target |
Other markers: 18S (eukaryotes), ITS (fungi), shotgun metagenomics (full functional + taxonomic profiling).
OTU vs ASV
| Feature | OTUs (97%) | ASVs | |---------|-----------|------|
[Description truncada. Veja o README completo no GitHub.]