Receba novas skills de Dados e Análise toda segunda
1 email curto, só com as skills novas de Dados e Análise. 4 minutos de leitura, sem spam, cancela com 1 clique.
Você confirma o email no primeiro envio. Sem spam. Cancela com 1 clique.
Ler descrição completaRecolher descrição
scRNA-seq: Quality Control and Preprocessing
Pitfalls
Mitochondrial threshold too strict: pct_counts_mt < 5% discards real cells in metabolically active types (cardiomyocytes, hepatocytes). Always check the joint distribution with total counts.
Skipping var_names_make_unique(): duplicate gene symbols cause cryptic downstream errors.
Normalizing before QC filtering: low-quality cells bias normalization. Filter first.
HVG selection on raw counts: must use normalize
[Description truncada. Veja o README completo no GitHub.]
