TF Footprinting & Chromatin Accessibility
Background
ATAC-seq: Tn5 transposase cuts and tags open chromatin. Nucleosome-free regions (NFRs) are cut frequently; nucleosome-occupied DNA is protected.
Footprinting: A bound TF blocks Tn5 at the binding site → local depletion of cuts at the motif center flanked by elevated cuts on either side.
| Feature | TF-bound sites | Unbound accessible sites |
|---|---|---|
| Cuts at motif center | Depleted (footprint) | Elevated |
| Cuts flanki |
[Description truncada. Veja o README completo no GitHub.]