Chromatogram Analysis: Sanger Sequencing
Phred Quality Score Reference
| Q Score | Error Probability | Accuracy | Interpretation | |:-------:|:-----------------:|:--------:|---------------| | 10 | 1/10 | 90% | Poor — avoid | | 20 | 1/100 | 99% | Minimum acceptable | | 30 | 1/1,000 | 99.9% | High confidence | | 40 | 1/10,000 | 99.99% | Excellent |
Formula: Q = -10 × log₁₀(P_error)
Practical thresholds: Q<20 = trim/re-sequence; Q20-29 = acceptable; Q≥30 = high confidence.
Sanger Read
[Description truncada. Veja o README completo no GitHub.]