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bio-crispr-screens-in-vivo-screens

Designs and analyzes in vivo CRISPR screens in animal tumor models, organoids, and immune-cell adoptive transfers. Covers bottleneck math (250x cells/sgRNA requires ~25M cells implanted; impossible for most syngeneic models, forcing focused libraries), focused library design (Manguso 2017 Nature 547:413 immune screen; Chen 2015 tumor screens), CRISPR-StAR intrinsic-control screening (Uijttewaal 20

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Atualizado há 4 meses

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Version Compatibility

Reference examples tested with: MAGeCK 0.5.9+, MAGeCK-VISPR 0.5.6+, pandas 2.2+, numpy 1.26+.

Before using code patterns, verify installed versions match. If versions differ:

  • CLI: mageck --version
  • Reference focused libraries: Manguso 2017, Chen 2015, public Addgene aliquots

If code throws ImportError, AttributeError, or TypeError, introspect the installed package and adapt the example to match the actual API rather than retrying.

In Vivo CRISPR Screen Analys

[Description truncada. Veja o README completo no GitHub.]

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