BUSCO Status Interpretation Guide
Overview
BUSCO (Benchmarking Universal Single-Copy Orthologs) is the standard tool for assessing genome, transcriptome, and proteome completeness by searching for conserved single-copy orthologs from the OrthoDB database. Correct interpretation of BUSCO output is essential for genome quality assessment, comparative genomics, and publication-ready reporting. The most common analytical error is excluding Duplicated BUSCOs from completeness counts, which art
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