Cell-Free Protein Synthesis (CFPS)
System Selection Guide
| System | Best For | Yield | PTMs | Disulfides | Cost | |--------|----------|-------|------|------------|------| | E. coli extract | Rapid prototyping, prokaryotic proteins | High (100-400 μg/mL) | None | Poor (reducing) | Low | | E. coli PURE | Defined conditions, unnatural AAs | Medium (50-150 μg/mL) | None | Controllable | High | | Wheat germ | Eukaryotic proteins, membrane proteins | High (100-500 μg/mL) | Limited
[Description truncada. Veja o README completo no GitHub.]