CellRanger Processor Skill
Purpose
Provide Cell Ranger capabilities for 10X Genomics single-cell data processing including demultiplexing and alignment.
Capabilities
- BCL to FASTQ conversion
- Cell barcode demultiplexing
- UMI counting
- Feature barcode processing
- Aggregate sample analysis
- Loupe Browser file generation
Usage Guidelines
- Configure sample sheets accurately
- Validate cell counts against expectations
- Review QC metrics from web summary
- Aggregate samples for combined analysis
- Generate Loupe files for visualization
- Document reference versions
Dependencies
- Cell Ranger
- STARsolo
Process Integration
- Single-Cell RNA-seq Analysis (scrnaseq-analysis)
- Spatial Transcriptomics Analysis (spatial-transcriptomics)