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kegg-pathway-analysis

Guide to KEGG pathway enrichment for DEG results. Covers ORA vs GSEA, mandatory directionality splitting, KEGG organism codes, API failure handling with offline fallbacks, cross-condition comparisons, and answer-first reporting. Consult when running enrichment with clusterProfiler or gseapy.

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KEGG Pathway Enrichment Analysis Guide

Overview

KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway enrichment analysis identifies biological pathways that are statistically over-represented among differentially expressed genes. This guide covers the two main enrichment approaches (ORA and GSEA), critical workflow decisions such as splitting genes by directionality, tool selection between R clusterProfiler and Python gseapy, and strategies for handling the notoriously unreliable KEGG R

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