Opentrons Protocol API v2 for OT-2/Flex: Python protocols for pipetting, serial dilutions, PCR, plate replication; control thermocycler, heater-shaker, magnetic, temperature modules. Use pylabrobot for multi-vendor.
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Opentrons Integration — Lab Automation
Overview
Opentrons provides a Python-based Protocol API (v2) for programming OT-2 and Flex liquid handling robots. Protocols are structured Python files with metadata and a run() function that controls pipettes, labware, and hardware modules. All protocols can be simulated locally before running on physical hardware.