QIIME2 Microbiome Analyzer Skill
Purpose
Enable QIIME2 microbiome analysis for 16S rRNA profiling and diversity analysis.
Capabilities
- Demultiplexing and denoising (DADA2)
- Taxonomic classification
- Alpha diversity metrics
- Beta diversity analysis
- Differential abundance testing
- Phylogenetic analysis
Usage Guidelines
- Use DADA2 for denoising amplicon data
- Select appropriate taxonomic classifier
- Calculate diversity metrics with rarefaction
- Visualize beta diversity with ordination
- Test differential abundance appropriately
- Document pipeline parameters
Dependencies
- QIIME2
- DADA2
- phyloseq
Process Integration
- 16S rRNA Microbiome Analysis (16s-microbiome-analysis)