RNA-seq Analysis Differential Expression
When to Use
- Differential gene expression from RNA-seq count data
- Comparing expression normalization strategies (RPKM / TPM / DESeq2 median-of-ratios)
- Building and interpreting count matrices (genes × samples)
- Exploratory analysis: PCA, hierarchical clustering of samples
- Downstream interpretation: volcano plots, MA plots, GSEA / ORA
Quick Reference
RNA-seq Workflow
FASTQ → QC (FastQC/fastp) → Alignment (STAR/HISAT2) → C
[Description truncada. Veja o README completo no GitHub.]