scrna-seq-analysis
Single-cell RNA-seq analysis from count matrix to annotated cell types and trajectories.
Quick Reference
| Step | Tool | Key Parameter |
|---|---|---|
| Load 10x data | sc.read_10x_mtx() | var_names='gene_symbols' |
| QC filter | sc.pp.filter_cells/genes | min_genes=200, pct_counts_mt < 20 |
| Normalize | sc.pp.normalize_total | target_sum=1e4 |
| Log transform | sc.pp.log1p | — |
| HVG selection | sc.pp.highly_variable_genes | `n_to |
[Description truncada. Veja o README completo no GitHub.]