Explorar skills

Execute ENCODE ATAC-seq processing pipeline from FASTQ to peaks and signal tracks. Child of pipeline-guide. Provides stage-by-stage Nextflow execution with Docker containers and cloud deployment. Handles Tn5 transposase offset correction, mitochondrial read removal, nucleosome-free fragment selection, and TSS enrichment scoring. Use when users need to process ATAC-seq data following ENCODE standar

Execute ENCODE DNase-seq pipeline from FASTQ to hotspots and footprints. Child of pipeline-guide. Provides Nextflow execution with Docker and cloud deployment. Use when processing DNase-seq data, calling DNase hypersensitive sites, performing footprinting analysis. Trigger on: DNase-seq pipeline, DNase hypersensitive, DHS, Hotspot2, footprinting, DNase I, chromatin accessibility DNase.

Execute ENCODE RNA-seq pipeline from FASTQ to gene quantification and signal tracks. Child of pipeline-guide. Provides Nextflow execution with Docker and cloud deployment. Use when processing RNA-seq data with STAR alignment, RSEM/Kallisto quantification, or generating expression matrices. Trigger on: RNA-seq pipeline, gene expression, STAR alignment, RSEM quantification, transcript quantification

Execute ENCODE ATAC-seq processing pipeline from FASTQ to peaks and signal tracks. Child of pipeline-guide. Provides stage-by-stage Nextflow execution with Docker containers and cloud deployment. Handles Tn5 transposase offset correction, mitochondrial read removal, nucleosome-free fragment selection, and TSS enrichment scoring. Use when users need to process ATAC-seq data following ENCODE standar

Execute ENCODE Hi-C pipeline from FASTQ to contact matrices and loop calls. Child of pipeline-guide. Provides Nextflow execution with Docker and cloud deployment. Use when processing Hi-C data, generating contact matrices, calling loops or TADs. Trigger on: Hi-C pipeline, chromatin conformation, contact matrix, loop calling, TAD detection, Juicer, HiCCUPS, 3D genome.

Execute CUT&RUN processing pipeline from FASTQ to peaks and signal tracks. Child of pipeline-guide. Provides Nextflow execution with Docker and cloud deployment. Use when processing CUT&RUN or CUT&Tag data, an alternative to ChIP-seq with lower background. Trigger on: CUT&RUN pipeline, CUT&Tag, SEACR, Henikoff, targeted chromatin, pA-MNase, process CUT&RUN.

Execute ENCODE ChIP-seq processing pipeline from FASTQ to peaks and signal tracks. Child of pipeline-guide. Provides stage-by-stage Nextflow execution with Docker containers and cloud deployment. Use when users need to process ChIP-seq data following ENCODE standards, run peak calling with MACS2, perform IDR analysis, or generate signal tracks. Trigger on: ChIP-seq pipeline, run ChIP-seq, process

Deploy NLWeb to production — Azure App Service (`deploy_azure_webapp.sh` + AI Search + Azure OpenAI), Snowflake Container Services, Cloudflare Worker + AutoRAG, Docker, and self-hosted. Covers env-var conventions, `mode: production` lockdown, scaling, TLS, OAuth, and CI for data reloads. Use when going from local dev to a hosted, internet-facing NLWeb instance.

Install WooCommerce, configure the development stack, and set up a local dev environment with WP-CLI, Docker, or wp-env. Use when setting up a new WooCommerce project or development environment.

Real-time cost tracking and budget management for Claude Code sessions. Shows current spend, burn rate, budget status, per-tool and per-model breakdown. Use when user says 'cost', 'budget', 'spending', 'how much', 'token usage', 'export', or '/cost-guardian'. Subcommands: budget, resume, reset, config, export, status.

Advanced cost intelligence: efficiency scoring, waste detection, ROI metrics, model savings calculator, anomaly detection, and GitHub badge generation. Use when user says 'insights', 'efficiency', 'waste', 'roi', 'savings', 'anomaly', 'badge', or '/cost-insights'.

Implanta aplicativos web, APIs ou agentes na internet com uma auditoria rigorosa pré-implantação e um fluxo de pré-visualização para produção. Ele detecta automaticamente vários tipos de projetos e os direciona para plataformas como Vercel, Railway ou Docker+SSH VPS, também suportando a execução local.