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Ultra-fast RNA-seq transcript/gene quantification via quasi-mapping (no BAM). Builds a k-mer index from transcriptome FASTA, quantifies in minutes. Outputs TPM/count tables (quant.sf) with optional GC- and sequence-bias correction. Integrates with tximeta/tximport for DESeq2/edgeR. Use STAR when a genome-aligned BAM is needed.

Retrieves biological sequences (DNA, RNA, protein) from NCBI and ENA with gene disambiguation, accession type handling, and comprehensive sequence profiles. Creates detailed reports with sequence metadata, cross-database references, and download options. Use when users need nucleotide sequences, protein sequences, genome data, or mention GenBank, RefSeq, EMBL accessions.

Gera documentação abrangente da arquitetura C4 para um repositório/base de código existente, utilizando uma abordagem de análise bottom-up.

Find duplicate or near-duplicate images in FiftyOne datasets using brain similarity computation. Use when users want to deduplicate datasets, find similar images, cluster visually similar content, or remove redundant samples. Requires FiftyOne MCP server with @voxel51/brain plugin installed.

Análise de desempenho full-stack, padrões de otimização e estratégias de monitoramento.

When the user wants to create or optimize in-app paywalls, upgrade screens, upsell modals, or feature gates. Also use when the user mentions "paywall," "upgrade screen," "upgrade modal," "upsell," "feature gate," "convert free to paid," "freemium conversion," "trial expiration screen," "limit reached screen," "plan upgrade prompt," or "in-app pricing." Distinct from public pricing pages (see page-

Analyze matchups, injuries, weather, Vegas lines. Recommend sit/start decisions with confidence levels for NFL, NBA, MLB, NHL, soccer.

Gerencia traduções i18n com Localhero.ai. Utilize ao trabalhar com arquivos de tradução, adicionar strings visíveis ao usuário ou modificar textos da interface.

Apply DDD tactical patterns in code using entities, value objects, aggregates, repositories, and domain events with explicit invariants.

Master on-call shift handoffs with context transfer, escalation procedures, and documentation. Use when transitioning on-call responsibilities, documenting shift summaries, or improving on-call processes.

Comprehensive systems biology and pathway analysis using multiple pathway databases (Reactome, KEGG, WikiPathways, Pathway Commons, BioModels). Performs pathway enrichment, protein-pathway mapping, keyword searches, and systems-level analysis. Use when analyzing gene sets, exploring biological pathways, or investigating systems-level biology.

React and Next.js performance optimization guidelines from Vercel Engineering. This skill should be used when writing, reviewing, or refactoring React/Next.js code to ensure optimal performance patterns. Triggers on tasks involving React components, Next.js pages, data fetching, bundle optimization, or performance improvements.