bg-szy

Identifies differential m6A methylation between conditions from MeRIP-seq paired IP/input data using exomePeak2 with `bam_ip` + `bam_input` (control arm) and `bam_treated_ip` + `bam_treated_input` (treatment arm) for integrated GC-bias-aware differential calling (Liu 2022 *NAR Genom Bioinform* 4:lqac046), QNB beta-binomial test (Liu 2017 *BMC Bioinformatics* 18:387), MeTDiff HMM-based differential

Enforce RED-GREEN-REFACTOR cycle. Use when implementing features, fixing bugs, or writing any production code.

Markdown linting and automated fixing using markdownlint-cli2. Use when Claude needs to: (1) Check markdown files for style issues, (2) Fix markdown formatting problems, (3) Ensure markdown follows best practices, (4) Validate markdown documents, or (5) Apply consistent markdown styling

When the user wants to create or optimize in-app paywalls, upgrade screens, upsell modals, or feature gates. Also use when the user mentions "paywall," "upgrade screen," "upgrade modal," "upsell," "feature gate," "convert free to paid," "freemium conversion," "trial expiration screen," "limit reached screen," "plan upgrade prompt," or "in-app pricing." Distinct from public pricing pages (see page-

When the user wants to create, optimize, or audit a feedback or roadmap page. Also use when the user mentions "feedback page," "roadmap," "feature requests," "vote on features," "Canny," "UserVoice," or "product feedback." For sitewide page planning, use website-structure.

Princípios de desenvolvimento de jogos multiplayer, incluindo arquitetura, rede e sincronização.

Guide for creating high-quality MCP (Model Context Protocol) servers that enable LLMs to interact with external services through well-designed tools. Use when building MCP servers to integrate external APIs or services, whether in Python (FastMCP) or Node/TypeScript (MCP SDK).

AI-powered molecular glue discovery for targeted protein degradation, enabling neo-substrate recruitment and undruggable target degradation through E3 ligase interface modulation.

Detect and analyze adverse drug event signals using FDA FAERS data, drug labels, disproportionality analysis (PRR, ROR, IC), and biomedical evidence. Generates quantitative safety signal scores (0-100) with evidence grading. Use for post-market surveillance, pharmacovigilance, drug safety assessment, adverse event investigation, and regulatory decision support.

Monitors Claude's responses for struggle signals and suggests escalation to deep-thinking agents when complexity exceeds comfortable reasoning capacity.

Find homologous sequences using iterative BLAST (PSI-BLAST), profile HMMs (HMMER), and reciprocal best hit analysis. Use when identifying orthologs, distant homologs, or protein family members where standard BLAST is not sensitive enough.

Comprehensive analysis of liquid biopsy data (ctDNA, CTCs) for cancer detection, MRD monitoring, and response tracking.