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TOON (Token-Oriented Object Notation) encoding for LLM-efficient data representation. 30-60% token savings vs JSON for structured data.

Git workflow management including branch validation, commit conventions, PR preparation, and repository health checks.

Laboratory automation toolkit for controlling liquid handlers, plate readers, pumps, heater shakers, incubators, centrifuges, and analytical equipment. Use this skill when automating laboratory workflows, programming liquid handling robots (Hamilton STAR, Opentrons OT-2, Tecan EVO), integrating lab equipment, managing deck layouts and resources (plates, tips, containers), reading plates, or creati

Python image processing for microscopy and bioimage analysis. Read/write images, filter (Gaussian, median, LoG), segment (thresholding, watershed, active contours), measure region properties, detect features. SciPy/NumPy ecosystem. Use OpenCV for real-time video; CellPose for DL cell segmentation; napari for visualization.

Prepares statistical reports for clinical trials following CONSORT 2025, SPIRIT 2025, ICH E9(R1) estimands, and FDA 2023 covariate adjustment guidance. Covers Table 1 generation, analysis populations (ITT/FAS/PP/Safety), the 5 ICH E9(R1) intercurrent-event strategies, MMRM under MAR (mmrm), reference-based MI (rbmi J2R/CR/CIR), Permutt tipping-point sensitivity, and Rubin's-rules vs frequentist va

Extract, process, and deduplicate reads using Unique Molecular Identifiers (UMIs) with umi_tools. Use when library prep includes UMIs and accurate molecule counting is needed, such as in single-cell RNA-seq, low-input RNA-seq, or targeted sequencing to distinguish PCR from biological duplicates.

Laravel architecture patterns, routing/controllers, Eloquent ORM, service layers, queues, events, caching, and API resources for production apps.

Perform in silico gene knockout analysis and synthetic lethality screens using COBRApy single and double deletions. Predict essential genes and identify synthetic lethal pairs for drug target discovery. Use when identifying essential genes or finding synthetic lethal drug targets.

This skill should be used when the user asks to "optimize context", "reduce token costs", "improve context efficiency", "implement KV-cache optimization", "partition context", or mentions context limits, observation masking, context budgeting, or extending effective context capacity.

End-to-end testing with Playwright — generate, run, and debug E2E tests.

Uma skill de fluxo de trabalho de desenvolvimento backend que impõe a nomeação de branches/PRs vinculados ao ClickUp, higiene de PRs, migrações seguras do Django e alterações de esquema sem tempo de inatividade para backends estilo Django4Lyfe.

Expert in strict POSIX sh scripting for maximum portability across