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Provide comprehensive clinical interpretation of somatic mutations in cancer. Given a gene symbol + variant (e.g., EGFR L858R, BRAF V600E) and optional cancer type, performs multi-database analysis covering clinical evidence (CIViC), mutation prevalence (cBioPortal), therapeutic associations (OpenTargets, ChEMBL, FDA), resistance mechanisms, clinical trials, prognostic impact, and pathway context.

Designs cytosine (CBE, C-to-T) and adenine (ABE, A-to-G) base-editor guides by positioning the target base at the activity-peak of the editing window (protospacer positions ~5-7, PAM-distal numbering), minimizing bystander edits for product purity, reading dinucleotide context (APOBEC1 TC favored / GC disfavored), and selecting the editor variant (BE4max, ABEmax, ABE8e, YE1/SECURE, TadCBE, CGBE, S

Go performance optimization techniques including profiling with pprof,

Select restriction enzymes by criteria using Biopython Bio.Restriction. Find enzymes that cut once, don't cut, produce specific overhangs, are commercially available, or have compatible ends for cloning. Use when selecting restriction enzymes for cloning or analysis.

End-to-end CRISPR screen analysis from FASTQ to hit genes. Orchestrates guide counting, QC, statistical analysis with MAGeCK, and hit calling with multiple methods. Use when analyzing pooled CRISPR screens from count data to hit calling.

Validates Conductor project artifacts for completeness,

Automated code review with security scanning, quality metrics, and best practices analysis. Use when reviewing code for: (1) Security vulnerabilities and common attack vectors, (2) Code quality issues and maintainability concerns, (3) Performance bottlenecks and optimization opportunities, (4) Best practices and design patterns, (5) Test coverage and testing strategies, (6) Documentation quality a

Knowledge and utilities for creating animated GIFs optimized for Slack. Provides constraints, validation tools, and animation concepts. Use when users request animated GIFs for Slack like "make me a GIF of X doing Y for Slack."

Builds a custom lead scoring model for a business. Takes ICP definition, historical win/loss data, CRM export. Analyzes which attributes correlate with closed-won deals. Generates lead-scoring-model.md with scoring dimensions, point values, thresholds, CRM implementation guide, and validation methodology. Can also score a batch of current leads against the model.

Choose presentation-ready color palettes and font pairings for PPT/design tasks. Use when users ask for visual theme choices, brand-safe palettes, or font recommendations. Triggers include: 配色, 色板, 字体, color palette, font, PPT配色, 字体搭配.

Programmatic access to Google NotebookLM via the notebooklm-py CLI and Python API. Use this skill whenever the user wants to create notebooks, add sources (URLs, YouTube, PDFs, files), generate audio overviews/podcasts, videos, slide decks, quizzes, flashcards, infographics, reports, mind maps, or data tables from their research materials. Also use when the user mentions NotebookLM, wants to turn

Analyze test coverage gaps and generate tests to improve coverage. Use when improving test coverage, finding untested code, or writing missing tests.