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Designs and analyzes pooled prime-editor (PE) screens for installing precise genetic variants without bystander confounding. Covers pegRNA design with PRIDICT and PRIDICT2 (Mathis 2023/2024) for predicting per-pegRNA editing efficiency, pegRNA architecture (spacer + scaffold + PBS + RTT), PE2 / PE3 / PE3b / PEmax / PEAR variants, MOSAIC in situ saturation mutagenesis (Hsu JY et al 2024 bioRxiv), t

Electronic lab notebook API integration. Access notebooks, manage entries/attachments, backup notebooks, integrate with Protocols.io/Jupyter/REDCap, for programmatic ELN workflows.

Agile product ownership toolkit for Senior Product Owner including INVEST-compliant user story generation, sprint planning, backlog management, and velocity tracking. Use for story writing, sprint planning, stakeholder communication, and agile ceremonies.

Comprehensive quality control for flow cytometry and CyTOF data. Covers flow rate stability, signal drift, margin events, dead cell exclusion, and batch QC. Use when assessing acquisition quality or identifying problematic samples before analysis.

Consulte moléculas bioativas e dados de descoberta de medicamentos do ChEMBL. Pesquise compostos por estrutura/propriedades, recupere dados de bioatividade (IC50, Ki), encontre inibidores e realize estudos de SAR para química medicinal.

FastAPI web framework patterns. Triggers on: fastapi, api endpoint, dependency injection, pydantic model, openapi, swagger, starlette, async api, rest api, uvicorn.

Query protein-protein and gene interaction databases (STRING, BioGRID, IntAct, SIGNOR, Reactome, HuRI, HuMAP, OmniPath, ConsensusPathDB, DIP). Use when building PPI networks, choosing between physical vs functional vs genetic interactions, signed/directed vs undirected, high-throughput vs curated, picking confidence thresholds, aggregating across resources, or navigating license constraints. Encod

Uma estrutura de pontuação para test-kitchen cookoff e omakase-off, invocada na Fase 4 para avaliar implementações usando pontuação de 5 critérios. Não deve ser invocada diretamente, pois é chamada por cookoff/omakase-off.

Differential gene expression analysis (Python DESeq2). Identify DE genes from bulk RNA-seq counts, Wald tests, FDR correction, volcano/MA plots, for RNA-seq analysis.

Comprehensive framework for deep analysis of articles, papers, and long-form content using 10+ thinking models (SCQA, 5W2H, critical thinking, inversion, mental models, first principles, systems thinking, six thinking hats). Use when users want to: (1) deeply understand complex articles/content, (2) analyze arguments and identify logical flaws, (3) extract actionable insights from reading material

Derive security requirements from threat models and business context. Use when translating threats into actionable requirements, creating security user stories, or building security test cases.

Postgres performance optimization and best practices from Supabase. Use this skill when writing, reviewing, or optimizing Postgres queries, schema designs, or database configurations.