bg-szy

Processes eDNA metabarcoding from raw paired-end reads to species tables, navigating ASV (DADA2, UNOISE3) vs OTU (swarm v2) decision (Callahan 2017 vs Schloss multi-copy-16S critique), marker/primer choice (Leray COI, MiFish 12S, 515F/806R 16S, ITS2) with primer-specific bias, OBITools3 v3 command-name break (obi stats plural; .tar.gz taxonomy), tag-jumping with dual-indexing (Schnell 2015; NovaSe

Replace with description of the skill and when Claude should use it.

Designs genomics experiments so technical nuisance variation (batch, lane, plate, flow cell, operator, reagent lot, processing day) is balanced against the biological variable of interest and therefore estimable rather than confounded, using constrained sample-to-batch assignment (designit, OSAT), the confounder/mediator/collider distinction, and the principle that no post-hoc correction recovers

Detects m6A modifications from Oxford Nanopore direct-RNA-sequencing (ONT DRS) signal data using m6Anet (Hendra 2022 *Nat Methods* 19:1590; multiple-instance-learning neural network over DRACH 5-mer signal). Covers the required upstream pipeline (Dorado / Guppy basecalling -> minimap2 transcriptome alignment with `-ax map-ont -uf -k14 --secondary=no` -> nanopolish eventalign with `--scale-events -

Codified expertise for demand forecasting, safety stock optimization, replenishment planning, and promotional lift estimation at multi-location retailers. Informed by demand planners with 15+ years experience managing hundreds of SKUs. Includes forecasting method selection, ABC/XYZ analysis, seasonal transition management, and vendor negotiation frameworks. Use when forecasting demand, setting saf

Select and configure nonlinear solvers for f(x)=0 or min F(x). Use for Newton methods, quasi-Newton (BFGS, L-BFGS), Broyden, Anderson acceleration, diagnosing convergence issues, choosing line search vs trust region, and analyzing Jacobian quality.

Python data processing pipelines with modular architecture. Use when building content processing workflows, implementing dispatcher patterns, integrating Google Sheets/Drive APIs, or creating batch processing systems. Covers patterns from rosen-scraper, image-analyzer, and social-scraper projects.

Build, debug, and optimize Claude API / Anthropic SDK apps. Apps built with this skill should include prompt caching. TRIGGER when: code imports anthropic/@anthropic-ai/sdk; user asks to use the Claude API, Anthropic SDKs, or Managed Agents (/v1/agents, /v1/sessions, /v1/environments). DO NOT TRIGGER when: code imports `openai`/other AI SDK, general programming, or ML/data-science tasks.

Optimize content for search engines with keyword analysis, readability scoring, meta descriptions, and competitor comparison. Use this when users want to improve SEO, optimize blog posts, or analyze content for search performance.

AI-powered analysis of long-read sequencing data (PacBio, ONT) for structural variant detection, isoform discovery, epigenetic modifications, and de novo assembly.

Gerencia um pool de requisitos onde usuários submetem ideias/pontos de dor. A IA organiza, mescla e arquiva estes, auxiliando na filtragem para novas versões, impulsionada por pontos de dor sem agendamento prévio.

Direct REST API access to PubMed. Advanced Boolean/MeSH queries, E-utilities API, batch processing, citation management. For Python workflows, prefer biopython (Bio.Entrez). Use this for direct HTTP/REST work or custom API implementations.